Transgene description: 3 kbp promoter of X. laevis myl7 (MLC2) gene driving expression of Gal4. REMI
Xla transgenics
#92 LBRS-Venus Xla.Tg(CMV:LBRS:Venus)Ueno
Transgene description: CMV-LBRS-Venus, consists of a cytomegalovirus (CMV) promoter, 1-238 amino acids of human lamin B receptor (LBRS) and Venus in pCS2p+ (REMI)
#91 LBRS-EGFP Xla.Tg(CMV:LBRS:GFP)Ueno
Transgene description: CMV-LBRS-EGFP, consists of a cytomegalovirus (CMV) promoter, 1-238 amino acids of human lamin B receptor (LBRS) in pEGFP-N3 (Clontech) (REMI)
#88 EGFP-Mem-A (F2) Xla.Tg(CMV:memGFP)Ueno
Available as live animals
#89 EGFP-Mito-A Xla.Tg(Hsa.UBC:COX8A-eGFP)Ueno
Transgene description: CMV-COX8-EGFP, consists of a cytomegalovirus (CMV) promoter, mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (COX8) and EGFP in pCS2p+ (REMI)
#5 NBT-Katushka [Xla.Tg(tubb2b:Katushka;Gga.cryaa:eCFP)2EXRC]
Transgene description: 3481bp N-β-tubulin (NBT) promoter and 5’UTR driving expression of Katushka (TurboFP-C); chicken crystallin alpha A enhancer 2 (alphaCE2x6) driving CFP expression in lens and pronephros as marker of integration (I-Sce-I).
#41 FLK-1-GFP [Xla.Tg(kdr:GFP)Mead]
Transgene description: 2.5 kb X. laevis flk-1 promoter sequence driving eGFP with the first intron downstream as enhancer ( ~3.5kb). Made with Sleeping Beauty transposon system.
#87 GATA1-GFP [Xla.Tg(gata1:GFP)Mead]
Transgene description: gata1 promoter and first intron enhancer driving GFP, Sleeping beauty.
#86 Pax8-GFP [Xla.Tg(pax8:GFP)Ogino]
Transgene description: A GFP-flpe-kan cassette was introduced in-frame into a X. tropicalis fosmid clone, AOPZ250282 (EXRC), using homologous recombination technique. After the recombination, the kangene used as a selection marker was flipped out from the fosmid by arabinose induced Flpe recombinase.
#19 Nkx2-5:GFP [Xla.Tg(nkx2-5b:GFP)Mohun]
Transgene description: 8 kb of promoter of the X. laevis nkx2-5 gene driving expression of GFP (variant mt5). Mohun Lab Plasmid #2147. Contact Tim Mohun for the sequence as it differs from the published NCBI AF283102. The promoter of the second laevis Nkx2-5 allele was used here. It proved stronger than the original published one (Sparrow et al 2000 Dev. Biol. 227, p65 to p79.)
#21 MLC1v:EGFP [Xla.Tg(mlc3:eGFP)Mohun]
Transgene description: 8Kb of X. laevis mlc3 ( synonym = MLC1v ) gene driving expression of enhanced GFP (eGFP). Promoter sequence is NCBI AY289208. Plasmid linearised with PmeI was inserted. Select best expressing animals at NF stage 37, monitor to NF stage 45.
#101 SCL:GFP [Xla.Tg(Mmu.tal1:GFP)Patnt]
Transgene description: Mouse SCL +19 enhancer which contains a GATA-ETS-GATA core motif driving GFP
#39 UbiC-GFP [Xla.Tg(Hsa.UBC:GFP)Amaya]
Transgene description: 1.5 kb of human ubiqutin C promoter driving venus GFP (Amaya lab) (SI124)
#40 FLK-1:GFP [Xla.Tg(kdr:GFP)Mead]
Transgene description: 2.5 kb X. laevis flk-1 promoter sequence driving eGFP with the first intron downstream as enhancer ( ~3.5kb). Made with Sleeping Beauty transposon system.
#45 NBT-tauGFP [Xla.Tg(tubb2b:maptGFP)Amaya]
Transgene description: 3481bp Neural-β-tubulin (NBT) promoter and 5’UTR driving expression of tauGFP in the neural tissue.
#42 Cardiac actin (full gene)-GFP [Xla.Tg(actc1:GFP)Amaya]
Transgene description: The whole cardiac actin gene (~9 kb), with GFP genetrap vector (511)/ (275D) in the intron, plasmid number, EA537/EA535
#43 Xic-GFP [Xla.Tg(Xtr.cdknx:GFP)Papal]
Transgene description: 4.7 kb X. tropicalis p27 Xic1 promoter driving GFP
#2 Crystallin-GFP [Xla.Tg(cryga:eGFP)Amaya]
Transgene description: The crystallin-GFP construct was generated by subcloning a 2.2 kb fragment of the crystallin genomic DNA encompassing the promoter region (received from Robert Grainger), upstream of an enhanced version of the GFP.
#20 MLC2:GFP [Xla.Tg(myl2:GFP)Mohun]
Transgene description: 3 kb of promoter of the X. laevis MLC2 (myosin light chain) gene driving expression of GFP
#22 Cardiac actin:GFP [Xla.Tg(actc1b:GFP)Mohun]
Transgene description: 580 bp of promoter of the X. laevis Cardiac actin gene driving expression of GFP (variant mt5).
