Xla transgenics

#23 Smad3:EGFP [Xla.Tg(smad3:eGFP)^Mohun]

Transgene description: 1.5 kb of promoter of the X. laevis Smad3 gene driving expression of eGFP

#24 LURP1:EGFP [Xla.Tg(slurp1l:eGFP)^Mohun]

Transgene description: 5 kb of promoter of the X. laevis LURP-1 gene driving expression of EGFP.

#83 flk1-eGFP [Xla.Tg(kdr:eGFP)^Pec]

Transgene description: A genomic fragment containing 2.5 kb of kdr promoter sequence up to exon 2, including the complete intron 1 containing enhancer elements essential for the correct expression pattern, was isolated and cloned into pBSK(+) (Meadows et al., 2009 Dev 136, 1115-1125). Next, the eGFP cDNA fused to the SV40 polyA was inserted into exon 1 to drive reporter expression under the control of the Xla kdr (Flk1) promoter.

#213 LMO2- Katushka [Xla.Tg(Xtr.lmo2:Katushka;cryga:Venus)^EXRC]

Transgene description: 4775bp X. tropicalis LMO2 promoter driving expression of Katushka (TurboFP-C); marker for integration selection: γ-cry VenusGFP. X. tropicalis fosmid AOPZ 60793; scaffold 82: 386828-391603. ISce-I transgenesis.

#61 mpeg-EGFP [Xla.Tg(Dre.mpeg1:eGFP;cryga:mCherry)^Amaya]

Transgene description: Zebrafish mpeg-EGFP from Felix Ellet

#60 Xla.Tg(Gga.tal1:Venus;cryga:mCherry)^Amaya

Transgene description:chicken SCL+19(+6.1 to +10.9)enhancer and SV40 minimal promoter from pGL2-Promoter (Promega) driving Venus expression (from Rita Ferreira). Co-injected with a gamma-crystallin mCherry construct.

#29 Tubulin.alpha1:GFP [Xla.Tg(Rno.tuba1a:GFP)^Brown]

Transgene description: Rat tubulin α-1 promoter (1696bp of 5’flanking α-1 tubulin DNA, exon1 and the first intron) fused in frame to the GFP.

#64 Sox10-GFP [Xla.Tg(Sox10:GFP)]^Jpsj

Transgene description: Sox10:GFP-HS4-Isce1 transgene contains 4.7Kb of upstream sequence from the transcription factor sox10 (including part of the 5’ UTR). I Sce-I

#6 FLH-GFP [Xla.Tg(Dre.noto:GFP)^Amaya]

Transgene description: Zebrafish flh promoter driving GFP, GenBank accession number AY214391. REMI

#17 FLH:GFP x Albino [Xla.Tg(Dre.noto:GFP)^1Amaya]

Transgene description: Zebrafish flh promoter driving GFP, GenBank accession number AY214391. REMI

#7 FLH-Gap43eGFP [Xla.Tg(Dre.noto:gap43-eGFP)^Amaya]

Transgene description: Zebrafish flh promoter driving Gap43eGFP (membrane bound form of GFP). GenBank accession number AY214391. REMI

#1 NBT-Katushka [Xla.Tg(tubb2b:Katushka;Gga.cryaa:eCFP)^1EXRC]

Transgene description: 3481bp N-β-tubulin (NBT) promoter and 5’UTR driving expression of Katushka (TurboFP-C); chicken crystallin alpha A enhancer 2 (alphaCE2x6) driving CFP expression in lens and pronephros as marker of integration (I-Sce-I).

#202 NBT-Katushka γ-cry-Venus [Xla.Tg(tubb2b:Katushka;cryga:Venus)^EXRC]

Transgene description: 3481bp of tubb2b (N-β-tubulin, NBT) promoter and 5’UTR driving expression of Katushka (TurboFP-C); marker for integration selection: γ-cry Venus. (I Sce-I)

#206 Pax3-Katushka [Xla.Tg(pax3:Katushka;cryga:Venus)^EXRC]

Transgene description: 2916bp X. laevis Pax3 promoter from Roberto Mayor (Mauricio Moreno)driving expression of Katushka (TurboFP-C); marker for integration selection: γ-cry VenusGFP. I Sce-I.

#106 Pax3-GFP Xla.Tg(pax3:GFP)^EXRC

Transgene description: 2916bp X. laevis pax3 promoter (from Roberto Mayor (Mauricio Moreno)) driving expression of GFP. Tol2

#30 Elastase:GFP [Xla.Tg.(Rno.elas:GFP)^Brown]

Transgene description: 205 bp rat elastase promoter driving GFP in the pancreas during metamorphosis (in the cytoplasm of the acinar cells of NF54 tadpoles). REMI

#25 Elas:TRDN-GFP3 [Xla.Tg.(Rno.elas:DNthra-GFP;cryga:GFP)^Brown]

Transgene description: 205 bp rat elastase promoter driving dominant negative (TRDN) form of the TRα (thyroid hormone receptor α) fused to GFP. REMI

#48 CAG-Venus [Xla.Tg(CAG:Venus)^Ueno]

Transgene description: The CAG-Venus plasmid consists of the Venus variant of YFP reporter cloned into the pCAGGS expression vector (Nagai et al., 2002; Sakamaki et al., 2005). pCAGGS drives expression under the control of the CAG promoter [human cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter], and is followed by the rabbit β-globin polyA signal sequence (Niwa et al., 1991). REMI

#33 CMV:tdTomato (Tom3) [Xla.Tg(CMV:tdTomato)^Ryff]

Transgene description: CMV driving expression of the Tomato red fluorescent protein. I Sce-I. http://www.addgene.org/Gerhart_Ryffel Accession ID: 30530

#8 CMV GFP [Xla.Tg(CMV:GFP)^Brown]

Transgene description: The constitutive simian cytomegalovirus (CMV) IE94 promoter/enhancer driving GFP.